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recombinant neuraminidase n1  (Sino Biological)
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recombinant murine cxcl10 protein rmcxcl10  (PeproTech)
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precision plus proteintm unstained protein standard  (Bio-Rad)
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precision plus proteintm unstained standard  (Bio-Rad)
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western solubilization buffer  (Thermo Fisher)
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Thermo Fisher western solubilization buffer
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murine recombinant stem cell factor (scf)  (PeproTech)
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bit-9500  (STEMCELL Technologies Inc)
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coomassie brilliant blue  (Bio-Rad)
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proteintech ift88  (Proteintech)
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Proteintech proteintech ift88
Figure 3. IFT 88/70/52/46 complex directly interacts with HSET, and depletion of <t>IFT88</t> reduces HSET turnover on mitotic spindle microtubules.
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puregene-prestained protein ladder 10–250 kda  (Genetix Biotech)
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Genetix Biotech puregene-prestained protein ladder 10–250 kda
Figure 3. IFT 88/70/52/46 complex directly interacts with HSET, and depletion of <t>IFT88</t> reduces HSET turnover on mitotic spindle microtubules.
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Image Search Results


Figure 3. IFT 88/70/52/46 complex directly interacts with HSET, and depletion of IFT88 reduces HSET turnover on mitotic spindle microtubules.

Journal: EMBO reports

Article Title: IFT proteins interact with HSET to promote supernumerary centrosome clustering in mitosis.

doi: 10.15252/embr.201949234

Figure Lengend Snippet: Figure 3. IFT 88/70/52/46 complex directly interacts with HSET, and depletion of IFT88 reduces HSET turnover on mitotic spindle microtubules.

Article Snippet: Proteintech: IFT88 (13967-1-AP; WB: 1/500; IF: 1/250), IFT52 (17534-1-AP; WB: 1/500; IF: 1/500), IFT81 a 2020 The Authors EMBO reports 21: e49234 | 2020 11 of 15 D ow nloaded from https://w w w .em bopress.org on A pril 5, 2024 from IP 2804:1b3:60c2:f68:e5e7:9006:acb2:f6da. (11744-1-AP; WB: 1/250), IFT140 (14460-1-AP; WB: 1/500); Novus Biological: IFT27 (NBP1-87170, WB: 1/200); Sigma-Aldrich: atubulin (T6169, WB: 1/1,000; IF: 1:1,000), FITC-conjugated a-tubulin (F2168, IF: 1/300), GAPDH (G8795, WB: 1/10,000); Abcam: a-GFP-FITC (ab6662, IF: 1/500), and HSET/KIFC1 (ab55388, WB: 1/1,000); Cell Signaling Technology: KIFC1/HSET (12313, IP: 1/100); Millipore: centrin (20H5, IF: 1/5,000); Homemade: c-tubulin (HM2569, gift from S. Doxsey laboratory [54], IF: 1/500); Homemade: polyglutamylated tubulin (GT-335, gift from C. Janke Lab [55]) and DAPI (Cell Signaling, IF 1/10,000).

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